The fluorescent microscope is a conventional light microscope and belongs to the family of compound microscopes but uses a higher intensity of light. It works two ways; it magnifies the sample and causes the sample to emit a glow or luminescence. What you see through the lens is a palette of colors or combination of colors.
Luminescence Art
The fluorescent microscope is an effective instrument for studying cells and invisible to the eye organisms; this is a standard microscope for scientific research, biology, and other sciences or industries that require a study of specimens that cannot be scrutinized by the unaided human eye. For accuracy of observation, the specimen is coated with a green fluorescent protein known as the fluorophore. When light hits the specimen, it emits a long wavelength of light of a different color.
Imagine yourself in the dark and watching glow in the dark objects. That s the closest thing description to the experience of peering through the fluorescent microscope. Typically, the specimen will give off different colors as sections of it will respond different to the stimulus. It is not unusual to see different patterns and different colors. In the study of a dividing cancer cell, researchers use different color stains on the specimen. Through the eyepiece you can observe unusual shapes in green or red.
Different samples from different specimens show different structures. Some you would say resemble cubic art. The shapes range from strands of filament, globules or circles and against a canvass of dark background , the specimens give an ethereal glow. Imagine a huge luminous painting. If these were not cancer cells or cells from sick cows, it would make a good art form. Perhaps somebody out there can imitate life in art, not the other way around.
Luminescence and Scientific Research
The fluorescent microscope is instrumental in the discovery of cures for diseases. Research studies are able to study specimens of diseased and healthy cells and their behavior or reaction to different stimulus. To get a specific wavelength for the emitted luminescence, excitation filters are used. This is positioned in the excitation path before the dichroic mirroic. The emission filter is used to select wavelength of the emission and this is placed under the dichroic mirror
For everybody s enlightenment, a dichroic mirror is a color filter that reflects colors. Used in conjunction with a light source the filter produces light perceived by the human as intense colors. The technique is popular in theatrical art. The amazing stage lights are created by filters to produce a desired effect to match the emotions played out on the stage. The same is reflected on the stage of the fluorescent microscope.
This special mirror is also transparent to heat, therefore allowing visible light to be aimed where needed and this transparency allows infrared heat waves to exit through the mirror s backside. This explains why the illumination produced more intense colors but less heat. To sum it up, fluorescence improves research methodologies for cellular biology, immunology, medical research, microbiology and other related fields.
Researchers can observe the different behaviors or characteristic of cells, thanks to the invented different stains and this microscope. Scientific research will continue to discover cures for different diseases. As you read this, much work is still done to improve the fluorescent microscope. Expect better and more exciting microscopy illumination as this microscope becomes more powerful.
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